BIOCHEMISTRY AND HUMAN PHYSIOLOGY LAB VIVA VOCE QUESTIONS


Department of Biomedical Engineering


BIOCHEMISTRY AND HUMAN PHYSIOLOGY LAB 
VIVA VOCE QUESTIONS


  1. Biological importance of Carbohydrate
  2. What is the difference between reducing and non reducing sugar?
  3. What is the confirmation test for monosaccharide?
  4. What is the significance of osazone?
  5. Biological importance of Lipids
  6. Difference between essential and nonessential amino acids
  7. Give eg for semi essential amino acids
  8. What is meant by Partition coefficient?
  9. Basic principle of Chromatography
  10.  What is the choice of stationary phase and mobile phase in  
  1. Basic principle of Electrophoresis
  2. What is the normal value of hemoglobin in women?
  3. List out the normal value of Lipid profile
  4. List out the risk factor for atherosclerosis
  5.  What is meant PCV?
  6. What is meant by MCH, MCV?
  7. What is the role PMNL?
  8. What is the level of fasting blood glucose?
  1. What is the difference between ketose and aldose sugar?
  2. What is the use of blank in colorimetric estimation?
  3. How to prepare a 4N solution?
  4.  What is normality, molality, molarity?
  5. Define Beer – Lamberts law
  6. Give an eg for basic amino acids
  7. What is the normal concentration of blood cholesterol?
  8. What is meant by oligosaccharide ? give eg
  9. What is meant by polysaccharide ? give eg
  10. What is meant by monosaccharide ? give eg
  11. What is meant by ketogenic amino acid ?
  12. What is meant by glucogenic amino acid ?
  13. Give examples for glucogenic and ketogenic aminoacid
  14. classification of carbohydrates
  15. classification of Lipids
  16. classification of Proteins
  17.  classification of amino acids
  18. lipoprotein classification and its function
  19. Define enzyme
  20. Define active site
  21. Define specificity of enzymes
  22. Give examples for allosteric regulation of enzymes
  23. Give examples for covalent modification of enzymes
  24. Give examples for feed back regulation of enzymes
  25. Give examples for hormonal regulation of enzymes
  26. List out the clinical significances of SGPT
  27. List out the clinical significances of SGOT
  28. List out the clinical significances of CPK
  29. List out the clinical significances of LDH
  30. Define electrophoresis
  31. Define isoelectric foccusing
  32. What is meant by PAGE?
  33. Define chromatography
  34. What is meant by partition coefficient?
  35. What is the role of mobile and stationary phase in chromatography?
  36. Give the principle of adsorption
  37. Principle and application of  gel electrophoresis
  38. Give the normal value of the following
a)    Blood Glucose
b)   Total Protein
c)    Serum Albumin, Globulin, Fibrinogen                                                                                                                                                                                                                                   
d)   Albumin to globulin ratio
e)    Total Cholesterol, LDL, HDL
f)     Lipid Profile
g)    Urine Creatinine
h)   Haemoglobin
i)     Erythrocyte sedimentation rate
j)     PCV, MCH, MCV, MCHC, CI




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